Chlamydia Trachomatis NAAT Culture

Test Name	Chlamydia trachomatis NAAT
Alternate Name(s)	(Information Unavailable)
Laboratory Module	Microbiology
Ordering Mnemonic	CHLAM
Specimen Type	First-void urine, Endocervical or cervix, Female Vaginal, Rectal, Pharyngeal
Collection Container	Endocervical/Urethral swab:) Roche cobas® PCR Media Dual Swab Sample Kit Urine: Roche cobas® Urine Sample Kit
Container Information	Container Unisex swab Urine
Collection Information	Note:Medico-legal investigations: CT and NG culture is the preferred and recommended method for medico-legal investigations; however, NAAT specimens will also be accepted. A positive NAAT result will be confirmed by another NAAT using a different set of primers as per the current Public Health Agency of Canada (PHAC) Canadian Guidelines on Sexually Transmitted Infections. Specimens received on patients <14 years of age have not been validated by the manufacturer; however, they will be tested by PHO with a disclaimer added. Collect approximately 10-50 mL of first-void urine in a sterile container. Using the provided disposable pipette, transfer urine from the sterile container to the Roche cobas® PCR Urine Sample Kit until the fluid level is between the two black fill lines. Tightly re-cap the Roche cobas® PCR Urine sample tube, and invert 5 times to mix. Urine specimens must be transferred into the Roche cobas® PCR Urine sample tube immediately for stabilization. The Roche cobas® PCR Media Dual Swab Sample Kit contains two swabs: a flocked swab and a woven swab. Each swab is optimized and validated for testing of a specific genital/extragenital site. The flocked swab is used for endocervical specimens, and the woven swab is used for vaginal, rectal and pharyngeal specimens. Do not pre-wet swabs in Roche cobas® PCR Media or any other liquid before collection of any specimens. Primary swab specimen tubes with no swabs or with two swabs have not been collected according to the collection instructions and therefore will not be tested. For either swab type, lower the swab specimen into the tube until the visible scoreline on the swab is aligned with the tube rim. The tip of the swab should be just above and not submerged into the liquid prior to breaking the shaft at the dark scoreline. Discard the top portion of the swab. Specimens received with no swab in the sample kit will be cancelled. The presence of mucous in endocervical specimens may cause process delays. Mucous-free specimens are required for optimal test performance. For endocervical specimen collection, use the large woven swab provided in the Roche cobas® PCR Media Dual Swab Sample Kit or equivalent device to clean and remove excess mucous. Discard this swab after use. Use only the flocked swab for specimen collection. Specimens received with two swabs in the sample kit will be cancelled. Vaginal, rectal and pharyngeal specimens should be collected using the woven swab. Do not let the woven swab touch any surface before placing it into the sample kit. Do not use the flocked swab; these specimens will be cancelled.
Test Schedule	Send-Out Monday thru Friday
Routine Turnaround Time	Preliminary: 48 hours Final: 7 days
Stat Turnaround Time	Preliminary: 24 hours Final: 7 days
Reference Interval	(Information Unavailable)
Critical Values	Critical positive reports are communicated
Lab Process Notes	(Information Unavailable)
Storage and Transport	If the specimen cannot be transported directly to the laboratory, the swab should remain at ambient temperature and the urine should be placed in a fridge. Once in the laboratory the swab specimen should be stored at room temperature until processing and the urine should be placed in the fridge.
Test Referred To	Public Health Laboratories, Hamilton