Creutzfeldt-Jacob Disease (CJD)

Cruetzfeldt-Jakob Disease

14-3-3 Protein Assay, Abnormal Prion, Bovine spongiform Encephalopathy, CJD

CJD

Sterile CSF conical tubes

Tube 1- Glucose and protein

Tube 2- Microbiology (other tests)

Tube 3- Cell Count (Microbiology is x4 tubes collected)

Tube 4- Cell Count

The last tube collected will be processed for the cell count

Lumbar Puncture

Clean the puncture site with antiseptic solution and alcohol before needle insertion. Insert a needle with stylet at the L3-L4, L4-L5 or L5-LS interspace. When subarachnoid space is reached, remove stylet and spinal fluid will appear in the needle hub. Slowly drain CSF into sterile tubes.

Shunt Fluid

Clean the reservoir site with antiseptic solution and alcohol. Collect fluid through the reservoir unit and place it in a sterile tube.
Indicate method of collection on requisition.

Suggested Volumes:

Routine Culture (C&S) - 2 mL

Fungus Culture - 2 mL

Mycobacterium Culture - 2 mL

Virus Culture - 2 mL

CJD-clear atraumatic CSF taken from a living patient 2-3 mls minimum. Please provide the waybill # at the time of notification.

Note: always send the most turbid tube to Microbiology.

Note: Specimen description and total quantity will be recorded for the CSF

The supernate/extra specimen will be frozen in the -70'C freezer for approximately 6 months.

Freeze directly after the lumbar puncture at -20'C or lower until assay is run. Do not treat with formaldehyde or formic acid. The specimen will need to be considered as 'Hazardous Material'.

Ship only Monday to Wednesday on dry ice, in frozen state to:

Division of Host Genetics and Prion Disease

National Microbiology Laboratory

1015 Arlington Street

Winnipeg, Manitoba R3E 3R2

Please provide the waybill# at the time of notification

The specimen should be delivered to the laboratory STAT, with no delay and should not be transported through the automated delivery system.

If after hours and no call-back is initiated the specimen should be stored at room temperature in the STAT container.

Offsite, Reference Lab