Activated Protein C Resistance (APCR)

Citrated Plasma and EDTA whole blood

Blue- 5.0 mL for APCR

Lavender 5.0 mL for Factor 5 Leiden

Double spin plasma. Ship frozen on dry ice.

* LT Blue
Specimen Handling:
ALWAYS partially fill a DISCARD TUBE first.

Then collect blood in a LIGHT BLUE top tube (citrated).
Collect a FULL TUBE. Mix thoroughly by gentle inversion.
Dispose the DISCARD TUBE into the biohazardous container.

IMMEDIATELY centrifuge for 15 minutes at 3500 rpm.
Carefully pipette all PLATELET POOR plasma,
starting from the top of the plasma, into a clear labelled centrifuge tube taking care NOT TO DISTURB the buffy coat layer
(white layer of platelets and white cells directly above the red cells).
Cap centrifuge tube.

IMMEDIATELY centrifuge this plasma for another 15 minutes
at 3500 rpm. Pipette the PLATELET POOR plasma, starting from the top of the plasma into one (1) labelled clear 12x75 mm aliquot tube taking care NOT TO DISTURB the BOTTOM of the tube. DO NOT TRANSFER ANY CELLS.
Cap tightly and FREEZE IMMEDIATELY.
Storage: Frozen (-20°C)

Transportation: Frozen. Specimens

FACTOR 5 Leiden:

Sample Requirements:
*Lavender
Specimen Handling:
Collect blood. Mix thoroughly by gentle inversion.

To be received within 5 days.

Storage: Room temperature (18-27°C)

Transportation: Room Temperature

Plasma - Ship frozen on dry ice. Store -40'C.

EDTA whole blood ship at ambient temperature