Stool Culture - Vibrio species and Aeromonas species

Stool Culture - Vibrio species and  Aeromonas species

Vibrio species

Aeromonas species

Microbiology

STOOL

Stool

Para-Pak Enteric Plus - Enteric Plus Transport Media (ETM) 

• Avoid the use of mineral oil, bismuth, Milk of Magnesia, antacids, compounds containing Kaolin or barium prior to collection. Avoid contamination with urine.
• Have patient pass stool onto a clean, dry bedpan. Transfer a portion of stool containing mucous, blood or pus into enteric transport media. Do NOT use toilet paper to transfer stool.
• Select areas of stool that appear blood-stained, purulent or watery. Transfer this to enteric transport media (labeled Enteric Plus). Thoroughly mix 5 mL of stool with the 20 mL of fixative (1:4 ratio).
• A thorough patient history, including symptoms, time of onset, age, travel history, food consumption and antimicrobial treatment is necessary to determine which pathogens to look for. 
• Only one sample per day is permitted, all others will be rejected unless approval from the microbiology department is arranged.
• Inpatients (in hospital 72 hours or more) will not be processed.

Daily

As growth is detected and identified the information is made available to the health care provider.

Specimen must be received within 48hours of collection.

Specimen should be transported to the laboratory ASAP after collection in biohazard bag.

Specimen should be stored at 2-8°C after receipt in the laboratory.

On site, BGH laboratory