Monkeypox virus

Test Name Monkeypox virus
Alternate Name(s) Mpox virus
Laboratory Module Microbiology
Ordering Mnemonic

MPOX PCR

Specimen Type
  • Lesion
  • Lesion Fluid
  • Crust material or scab
  • Nasopharyngeal swab
  • Throat swab
  • Urine
  • CSF
Collection Container
  • Universal Transport Media (UTM) for Lesion, Lesion Fluid, crust material, scab, Nasopharyngeal and/or Throat swab
  • Sterile container for Urine
  • CSF sterile tube for CSF
Container Information

 UTM                                  Sterile Container              CSF sterile tube

           

Collection Information

Submit a maximum of three skin lesion specimens per patient – based on PHO’s laboratory data, detection sensitivity from individual skin specimens is high (approximately 90%) in patients with laboratory-confirmed monkeypox infection.

NP /Throat swabs and blood specimens are generally not recommended in patients who have skin lesions that can be swabbed

Urine is not considered a routine specimen for Mpox testing but may be considered for collection. Performance characteristics for this specimen type are not well understood by referral laboratory.

 Test Schedule Monday - Friday
Routine Turnaround Time

3-5 days

14-21 days for Positive or Indeterminate specimens 

Critical Values 

Positive result is made available to the healthcare provider

Lab Process Notes Approval for testing and notification of specimen submission is not required. Contact PHO Customer Service (416-235-6556/1-877-604-4567) or the on-call Duty Officer (416-605-3113) after hours if you wish to consult prior to sample collection and shipment
Storage and Transport

Specimen should be transported to laboratory as soon as possible after collection. 

Specimen should be stored at 2°C - 8°C after receipt in the laboratory and during transportation.

If transportation to referral laboratory delayed more than 72 hours, specimen should be frozen at -70°C.

 Effective July 25, 2022, clinical specimens from patients undergoing Monkeypox virus testing have been temporarily reclassified as UN3373 Biological Substance, Category B for land and air transport. In addition to the routine Category B requirement, the outer packaging must be marked, on a contrasting background, with “TU 0886”, “Temporary Certificate – TU 0886” or “Certificat Temporaire – TU 0886”. For full details on packaging and transporting, see the Temporary Certificate TU 0886.

Test Referred To  Offsite, Referral Laboratory 

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