Legionella Urine antigen

Legionella Antigen Urine Test

Test Name:  

Legionella Antigen Urine Test

Alternate Name (s):  


Laboratory Module:  


Ordering Mnemonic:  


For infection control surveillance purposes this procedure will default when an ICU patient has a urine culture ordered.

Specimen Type:   


Collection Container:  

Sterile Container


Collection Information:  

Clean catch or midstream urine-send first morning voided urine. Do not use disinfectants for cleaning.

Female-Thoroughly clean the urethral opening and vaginal vestibule area with soapy water or clean gauze pads soaked with liquid soap. Rinse the area well with sterile water or wet gauze wipes. Hold the labia apart during voiding. Allow a few milliliters of urine to pass. Do not stop the flow. Collect the midstream portion in a sterile container.

Male- Cleanse the penis, retract the foreskin (if not circumcised), and wash with soapy water. Rinse the area well with sterile water. Keeping the foreskin retracted, allow a few milliliters of urine to pass. Do not stop the flow. Collect the midstream portion in a sterile container.

Ileal Conduit Urine- Remove the external urinary appliance, and discard the urine within. Gently swab and clean the stomal opening with 70% alcohol pad and then with an iodine solution. Remove excess iodine with 70% alcohol after procedure. Using sterile technique, insert a double catheter into the stoma. Catheterize the ileal conduit to a depth beyond the fascial level. Collect the urine drained into a sterile container.

Catheter Urine (In/Out)- Prior to catheterization, the patient should force fluids until the bladder is full. Clean the urethral opening (an in females, the vaginal vestibule), with soap and carefully rinse the area with water. Using sterile technique, pass a catheter into the bladder. Collect the initial 15-30ml of urine and discard it from the mouth of the catheter. Collect the remaining sample into a sterile container.

Catheter (indwelling)- Clean the catheter collection port with 70% alcohol. Urine sterile technique , puncture the port with a needle attached to a syringe. Aspirate the urine and place it in a sterile container. Do not collect urine from the collection bag. 

Test Schedule:  


Turnaround Time:  


1 day

Stat / Urgent

1 day

Reference Interval:  


Critical Values:  

As antigen is detected and identified the information is made available to the health care provider.

Lab Process Notes :  


Storage & Transport:  

Refrigerate all samples until they can be transported quickly to the laboratory.

Test Referred to :   

Public Health Laboratories, Hamilton

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Last updated November 27, 2007


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