Amputation of limb

Test Name:   Amputation of limb
Alternate Name (s):    
Laboratory Module:   Pathology
Ordering Mnemonic:    
Specimen Type:   

A excised limb

Collection Container:   Place in plastic bag and cardboard box
Container Information
Histo ContainerHisto ContainerHisto ContainerHisto Container

All of these containers are pre-filled with 10% Neutral Buffered Formalin

Please select the most appropriate size of container for the specimen so that there is a ratio of tissue: formalin (1:15), this is key for proper fixation

Containers may be obtained through the stores department.

Collection Information:   Specimen should be collected in the operating room

I.1. All specimens must be accompanied by a completed Surgical Pathology Card (form PATH_6707_0103).

The following information is required on the card, clearly written in ink:
(a) Patient identifiers
     (i) patient's first and last name
     (ii) unique number
     (iii) date of birth
     (iv) Ontario Health Insurance Number with version code.
(b) Submitting area identifiers: Hospital site (BGH or The Willett) and the submitting department (Emergency, Ambulatory Care etc.).
(c) Submitting physician's name.
(d) Date of surgery
(e) Pre- and post-operative presumptive diagnosis.
(f) A brief clinical history.
(g) Specimen information
     (i) tissue type (ie. Breast)
     (ii) anatomical site (ie. Right side)
     (iii) surgical procedure (ie. wire localization biopsy)

I.2. The specimen container must be labeled. Addressograph labels are preferred, otherwise the following information must be provided, clearly written in ink:
(a) Patient identifiers
    (i) patient's full name
    (ii) unique number
    (iii) and at least one other unique additional identifier (ie. date of birth, OHIN).
(b) Date of surgery.
(c) Specimen name, anatomic site and surgical procedure, as it is written on the Surgical Pathology card.
This information must be recorded on the side of the specimen container and not the lid.
If a specimen is known or suspected to contain unique or extreme biohazard (e.g. CJD) the container shall be so marked.

I.3. Transport specimen to the morgue

I.4. Inform histology staff that you have delivered a specimen to the morgue by delivering the surgical card to the histology department


Test Schedule:  

Monday to Friday

Turnaround Time:   Routine

1 week

Stat / Urgent

2 days

Reference Interval:  


Critical Values:    
Lab Process Notes :    
Storage & Transport:   Specimen should be stored in the morgue refrigerator and the surgical card brought to pathology.
Test Referred to :    BGH, On site


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