Peritoneal/Paracentesis Effusion for Cytology

Test Name:   Peritoneal Effusion for Cytology
Alternate Name (s):   Paracentesis
Laboratory Module:   Cytology
Ordering Mnemonic:  

category - Cyto

Specimen Type:   

Peritoneal Fluid

Collection Container:   Collect in a sterile 90 ml container . Container must be heparinized (3ml fill in 6 ml Heparin Syringe 100 units/ml) and  topped up completely with fluid. Indicate that you have added heparin in the specimen container. Invert container 10 times to ensure adequate mixing. For smaller amounts calculate 3-5 IU of heparin per ml of fluid.
Container Information
Sterile Container
Collection Information:  


I.1.All specimens must be accompanied by a completed manual or Meditech generated cyto-pathology non-gynecological requisition. All mandatory fields must be satisfied in order to complete the request. In the case of computer downtime use the standard cytopathology non-gynecological requisition.

The following information is required on the requisition
(a) Patient identifiers (addressographs may be used)
      (i) patient's first and last name
      (ii) hospital unique number
      (iii) date of birth
      (iv) Ontario Health Insurance Number with version code.
(b) Submitting area identifiers: Hospital site (BGH or The Willett) and the submitting department (Emergency, Ambulatory Care etc.).
(c) Submitting physician's name.
(d) Date of specimen collection
(e) Pre- and post-operative presumptive diagnosis.
(f) A brief clinical history, relevant pathological and radiological findings.
(g) Specimen information
     (i) specimen type/source
     (ii) specimen site
I.2. If received, the specimen container must be labeled. Addressograph labels are preferred, otherwise the following information must be provided, clearly written in ink:
(a) Patient identifiers -
     (i) patient's full name
     (ii) hospital unique number
     (iii) and at least one other unique additional identifier (ie. date of birth, OHIN).
(b) Date of specimen collection.
(c) Specimen type and site, as it is written on the Cytology Requisition form.
This information must be recorded on the side of the specimen container and not the lid.
If a specimen is known or suspected to contain unique or extreme biohazard (e.g. CJD) the container shall be so marked.

I.3.I.3.Document total volume of fluid received on the cytology requisition

I.4. Place specimen containers in a biohazard bag (if applicable). The requisition should be placed in the outer pouch of the biohazard bag.

I.5. Transport specimen to the Triage area of the laboratory as soon as possible

I.6. Inform the triage staff that you have delivered a specimen.

1.7  For Supplies, call Histology ext 2456 (Monday-Friday 0600 hrs to 1600 hrs)


Test Schedule:  

Monday to Friday prior to 1:00 pm

Turnaround Time:   Routine

1 week

Stat / Urgent

2-3 days

Reference Interval:  


Critical Values:    
Lab Process Notes :   Refrigerate (4'C) up to 72 hours unfixed. If there is a delay in processing add equal volumes of cytology fixative. Refrigerate the whole specimen. Be sure to have indicated it is a cytology specimen by placing a pink cytology label on the bottle and if fixation is required be sure to label the container with the appropriate cytology fixative label.

The specimen description should be recorded including, colour, quantity and any other feature worth noting.

Specimens received with a volume of <10mls should have cytology fixative added

Storage & Transport:   Specimens should be stored in the refrigerator
Test Referred to :    BGH, On site
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